Protocol for in vitro fluorescence assay of papain-like protease and cell-based immunofluorescence assay of coronavirus infection

Here, we describe detailed steps to constitute an in vitro assay for monitoring papain-like protease of coronavirus and a cell-based immunofluorescence infection assay. These assays can be adapted for high-throughput screen to determine the efficacy of novel protease inhibitors of coronaviruses and other viruses. In addition, cell-based immunofluorescence infection assay can be used to visually analyze antiviral efficacy of any novel compounds. Graphical Publisher's note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Response to the coronavirus disease 2019 pandemic of dental hygienists in South Korea-Part III: COVID-19 vaccination intentions.

OBJECTIVES: This cross-sectional study was to determine the coronavirus disease 2019 (COVID-19) vaccination intention of clinical dental hygienists in South Korea and the factors that influence vaccination intention. METHODS: COVID-19 vaccination intention of the 500 participants was confirmed through a survey including the following options: 'I will vaccinate (VAC)', 'I will not vaccinate (NoVAC)' and 'I do not know if I should get vaccinated (UNK)'. A Chi-square test was performed to determine whether there were differences in COVID-19 vaccination intention according to the general characteristics of the participants, degree of infection control knowledge (Score-K) and practice (Score-P) in response to COVID-19, fears over COVID-19 (Fear-C) and the level of anxiety before (GADBefore ) and after (GADAfter ) the COVID-19 pandemic. Multiple logistic regression was performed to identify factors affecting VAC and NoVAC by setting the base category as UNK. The p-values of <0.05 were considered statistically significant. RESULTS: According to the analysis, 44.8%, 18.8% and 36.4% of participants selected VAC, NoVAC and UNK respectively. There were significant differences in vaccination intention according to age, monthly income, residential area, symptoms related to COVID-19, Score-K, Fear-C and GADBefore . Compared to UNK, < $2000 monthly income, Score-K and Fear-C variables significantly influenced the opinion of VAC. Compared to the answer UNK, monthly incomes of $2000 to $2360 and $2360 to $2730 in residential areas significantly influenced the opinion of NoVAC. CONCLUSIONS: The variables influencing vaccination intention were monthly income, residential area, Score-K and Fear-C.

Partitioning online experiential consumption increases subjective well-being during the times of uncertainty

The current study suggests an effective compensatory control strategy using partitioning experiential consumption that can be implemented into consumers' daily activities. Across four experiments, we examined the effects of partitioned (vs. aggregated) message formats and financial anxiety on sense of control, subjective well-being, and ad attitudes. Specifically, our findings indicated that consumers with higher (vs. lower) levels of financial anxiety respond more favorably toward partitioned (vs. aggregated) experiential messages. Our results provide timely contributions for researchers and practitioners who are interested in understanding and implementing advertising and marketing strategies in the digital landscape with improving consumers' well-being in mind. (PsycInfo Database Record (c) 2022 APA, all rights reserved)

Risk of SARS-CoV-2 infection in a large cohort of Ontario workers

IntroductionWork is a key determinant of COVID-19 outcomes, however occupational surveillance is a critical information gap in many countries, including Canada. Understanding the risk of SARS-CoV-2 by occupation can identify high risk groups that can be targeted for prevention strategies.Materials and MethodsThe cohort includes 1,205,847 former workers compensation (non-COVID-19) claimants (aged 15–65) linked to health databases in Ontario, Canada. Incident cases were defined as either having a confirmed positive polymerase chain reaction (PCR) test in the Ontario Laboratory Information System (OLIS), or an International Classification of Diseases (ICD-10-CA) diagnostic code of U07.1 in hospitalization or emergency department records (February 2020-December 2021). Workers were followed until diagnosis, death, emigration, age 65 or end of follow-up. Sex- and age-adjusted Cox proportional hazards models were used to estimate hazards ratios (HR) and 95% confidence intervals (CI) by occupation, compared to all other cohort members. Analyses were also conducted to examine occupational trends in testing and diagnosis during waves of infection.ResultsOverall, 80,740 COVID-19 cases were diagnosed among workers during follow-up, of those, 80% were diagnosed with a positive PCR test. Associations were identified between COVID-19 diagnosis and employment in nursing (HR=1.44, CI95%=1.40–1.49), air transport operating (HR=1.61, CI95%=1.47–1.77), textile/fur/leather products fabricating, assembling, and repairing (HR=1.38, CI95%=1.25–1.54), apparel and furnishing services (HR=1.38, CI95%=1.19–1.60), and janitor and cleaning services (HR=1.11, CI95%=1.06–1.16). Restricted analyses where health care workers were omitted from the comparison group strengthened some associations for other high-risk workers. Test positivity ranged between 4–16% across major occupation groups. Risks varied over time and with changes in protective measures in workplaces and in broader communities.ConclusionsElevated risk of SARS-CoV-2 infection in health care, manufacturing, transportation, and service workers were identified, underscoring the importance of including occupational data in COVID-19 surveillance. Occupational trends in severe outcomes and vaccination are also being explored.

Plasma cell myeloma initially diagnosed as light-chain deposition disease on liver biopsy: A case report and literature review.

RATIONALE: Light-chain deposition disease (LCDD) is a rare condition characterized by the abnormal deposition of monoclonal light chains (LCs) in multiple organs, leading to progressive organ dysfunction. Herein, we report a case of plasma cell myeloma initially diagnosed as LCDD on liver biopsy performed for prominent cholestatic hepatitis. PATIENT CONCERNS: A 55-year-old Korean man complained of dyspepsia as the main symptom. On abdominal computed tomography performed at another hospital, the liver showed mildly decreased and heterogeneous attenuation with mild periportal edema. Preliminary liver function tests revealed abnormal results. The patient was treated for an unspecified liver disease; however, his jaundice gradually worsened, prompting him to visit our outpatient hepatology clinic for further evaluation. Magnetic resonance cholangiography revealed liver cirrhosis with severe hepatomegaly of unknown cause. A liver biopsy was performed for the diagnosis. Hematoxylin and eosin staining revealed diffuse extracellular amorphous deposits in perisinusoidal spaces with compressed hepatocytes. The deposits, which morphologically resembled amyloids, were not stained by Congo red but stained strongly positive for kappa LCs and weakly positive for lambda LCs. DIAGNOSES: Therefore, the patient was diagnosed with LCDD. Further systemic examination revealed a plasma cell myeloma. INTERVENTIONS: Fluorescence in situ hybridization, cytogenetics, and next-generation sequencing tested in bone marrow showed no abnormalities. The patient initially received bortezomib/lenalidomide/dexamethasone as the treatment regimen for plasma cell myeloma. OUTCOMES: However, he died shortly thereafter because of coronavirus disease 2019 complications. LESSONS: This case demonstrates that LCDD may present with sudden cholestatic hepatitis and hepatomegaly, and may be fatal if patients do not receive appropriate and timely treatment because of delayed diagnosis. Liver biopsy is useful for the diagnosis of patients with liver disease of unknown etiology.

Rapid discovery and classification of inhibitors of coronavirus infection by pseudovirus screen and amplified luminescence proximity homogeneous assay.

To identify potent antiviral compounds, we introduced a high-throughput screen platform that can rapidly classify hit compounds according to their target. In our platform, we performed a compound screen using a lentivirus-based pseudovirus presenting a spike protein of coronavirus, and we evaluated the hit compounds using an amplified luminescence proximity homogeneous assay (alpha) test with purified host receptor protein and the receptor binding domain of the viral spike. With our screen platform, we were able to identify both spike-specific compounds (class I) and broad-spectrum antiviral compounds (class II). Among the hit compounds, thiosemicarbazide was identified to be selective to the interaction between the viral spike and its host cell receptor, and we further optimized the binding potency of thiosemicarbazide through modification of the pyridine group. Among the class II compounds, we found raloxifene and amiodarone to be highly potent against human coronaviruses including Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), and SARS-CoV-2. In particular, using analogs of the benzothiophene moiety, which is also present in raloxifene, we have identified benzothiophene as a novel structural scaffold for broad-spectrum antivirals. This work highlights the strong utility of our screen platform using a pseudovirus assay and an alpha test for rapid identification of potential antiviral compounds and their mechanism of action, which can lead to the accelerated development of therapeutics against newly emerging viral infections.

Being (Asian) American children: children's exploration of racial/cultural identity and racism

This study explores young Asian American children's emerging ideas of racial identity and experiences of racism through multimodal representations during the COVID-19 Pandemic. To explore children's identity and understanding of racial experience, we used Suda (수다) with children, which refers to deeply engaged conversations and often involves participants' emotions. Adopting autoethnography, we used multiple data sources, including documentation of children's conversations with family relating to their identity and experiences of racism and a collection of children's artifacts. Findings showed that our children explored their racial identity in various aspects surrounding them, ranging from their body, classroom materials, and passports to their family origins. Suda provided the space for our children to freely explore their racial identities and experiences of Asian racism/Hate Crime. [ FROM AUTHOR]

Chemical screen uncovers novel structural classes of inhibitors of the papain-like protease of coronaviruses.

The papain-like protease (PLpro) of coronaviruses is an attractive antiviral target to inhibit both viral replication and interference of the host immune response. We have identified and characterized three novel classes of small molecules, thiophene, cyanofuran, and triazoloquinazoline, as PLpro inhibitors. Thiophene inhibited the PLpro of two major coronaviruses, Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus (SARS-CoV) including SARS-CoV-2, while cyanofuran and triazoloquinazoline more selectively inhibited MERS-CoV PLpro. Unlike GRL0617, a known PLpro inhibitor, all three compounds contain no naphthyl group but like GRL0617 were predicted to fit on the cleft near the BL2 loop. Docking studies further revealed that the location and direction of the binding determined their specificity to different coronaviruses. Together, our work demonstrates that the BL2 loop and nearby regions are outstanding druggable targets, and our three inhibitors can be applicable to the development of therapeutics for coronavirus infection.

Travel patterns of free-floating e-bike-sharing users before and during COVID-19 pandemic.

Free-floating micro-mobility as a mobility solution is becoming increasingly popular in cities. In this study, the travel patterns of free-floating electric bike-sharing service (FFEBSS) users before and during the COVID-19 pandemic were explored using big data and data mining. Existing real-time data studies provide a limited understanding of trip patterns and the characteristics of each user. Interpretations concerning the occurrence of life-changing events such as the COVID-19 pandemic are important. This study aimed to understand each user over 13 months comprising multiple time frames of market trends, seasonal change, and the COVID-19 pandemic outbreak. Multiple features were extracted from each user to explain the hidden data characteristics, and a data mining method was employed for clustering and evaluating user similarities with the extracted features. The results showed that FFEBSS users demonstrated a moderately stable travel pattern despite the COVID-19 pandemic, indicating the possibility of micro-mobilities being well adoptedas our future urban transportation.

Partitioning online experiential consumption increases subjective well-being during the times of uncertainty

The current study suggests an effective compensatory control strategy using partitioning experiential consumption that can be implemented into consumers' daily activities. Across four experiments, we examined the effects of partitioned (vs. aggregated) message formats and financial anxiety on sense of control, subjective well-being, and ad attitudes. Specifically, our findings indicated that consumers with higher (vs. lower) levels of financial anxiety respond more favorably toward partitioned (vs. aggregated) experiential messages. Our results provide timely contributions for researchers and practitioners who are interested in understanding and implementing advertising and marketing strategies in the digital landscape with improving consumers' well-being in mind. (PsycInfo Database Record (c) 2022 APA, all rights reserved)

Chemical screen uncovers novel structural classes of inhibitors of the papain-like protease of coronaviruses

The papain-like protease (PLpro) of coronaviruses is an attractive antiviral target to inhibit both viral replication and interference of the host immune response. We have identified and characterized three novel classes of small molecules, thiophene, cyanofuran, and triazoloquinazoline, as PLpro inhibitors Thiophene inhibited the PLpro of two major coronaviruses, Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus (SARS-CoV) including SARS-CoV-2, while cyanofuran and triazoloquinazoline more selectively inhibited MERS-CoV PLpro. Unlike GRL0617, a known PLpro inhibitor, all three compounds contain no naphthyl group but like GRL0617 were predicted to fit on the cleft near the BL2 loop. Docking studies further revealed that the location and direction of the binding determined their specificity to different coronaviruses. Together, our work demonstrates that the BL2 loop and nearby regions are outstanding druggable targets, and our three inhibitors can be applicable to the development of therapeutics for coronavirus infection. Graphical

Feasibility of a Synchronous Virtual Hypertension Management Program for Community-Dwelling Older Adults Through Extension

Background Hypertension (HTN) represents a primary risk factor for many chronic diseases. Two-thirds of older adults have HTN with the lowest control rate. A system-level approach through a partnership with Extension can potentially tackle this disease. Also, synchronous virtual programs can provide flexible and interactive learning opportunities to older adults isolated during COVID-19. Objective To describe the feasibility of a virtual HTN management program and assess its efficacy in improving mediators of HTN-related behavior changes. Study Design, Settings, Participants This study was a non-controlled, pre-post experimental intervention implemented in (state). The virtual synchronous HTN program comprised of eight-week sessions was advertised and available via Zoom at three different time frames. Reflecting the realities of community participation, it was not mandatory for participants to attend all eight sessions. A total of 74 primarily hypertensive older adults aged ≥55 years old participated in the program and completed online pre and post-surveys. Measurable Outcome/Analysis: For each session, online pre and post-surveys adopted from a validated tool were used to assess key HTN-related constructs including self-efficacy, perceived benefits, and stages of behavior change. Descriptive statistics and paired t-test (or Wilcoxon signed-rank test) were used for analysis. Results A total of 74 older adults attended at least one session (three on average), and about 15 participants completed pre and post-surveys per session. Study findings presented increases in self-efficacy for desirable behaviors to manage HTN in the Grains (P = 0.012), Fruits & Vegetables (FV) (P = 0.024), Meats & Other Proteins (P = 0.035), and Fats & Sweets (P = 0.034) sessions. Also, the perceived benefits of modifying eating patterns was improved in Sodium (P = 0.004), Grains (P = 0.001), and Meats & Other Proteins (P = 0.034) sessions. Further, participants showed improved readiness to change for the Grains (P = 0.011) and FV (P = 0.046) sessions. Conclusions A synchronous virtual HTN program for hypertensive older adults is feasible and effective in improving key constructs related to HTN. Collaborative partnership with Extension is a promising approach to ensure program sustainability. Funding USDA.

Association between long-term exposure to ambient air pollution and COVID-19 severity: a prospective cohort study.

BACKGROUND: The tremendous global health burden related to COVID-19 means that identifying determinants of COVID-19 severity is important for prevention and intervention. We aimed to explore long-term exposure to ambient air pollution as a potential contributor to COVID-19 severity, given its known impact on the respiratory system. METHODS: We used a cohort of all people with confirmed SARS-CoV-2 infection, aged 20 years and older and not residing in a long-term care facility in Ontario, Canada, during 2020. We evaluated the association between long-term exposure to fine particulate matter (PM2.5), nitrogen dioxide (NO2) and ground-level ozone (O3), and risk of COVID-19-related hospital admission, intensive care unit (ICU) admission and death. We ascertained individuals' long-term exposures to each air pollutant based on their residence from 2015 to 2019. We used logistic regression and adjusted for confounders and selection bias using various individual and contextual covariates obtained through data linkage. RESULTS: Among the 151 105 people with confirmed SARS-CoV-2 infection in Ontario in 2020, we observed 8630 hospital admissions, 1912 ICU admissions and 2137 deaths related to COVID-19. For each interquartile range increase in exposure to PM2.5 (1.70 µg/m3), we estimated odds ratios of 1.06 (95% confidence interval [CI] 1.01-1.12), 1.09 (95% CI 0.98-1.21) and 1.00 (95% CI 0.90-1.11) for hospital admission, ICU admission and death, respectively. Estimates were smaller for NO2. We also estimated odds ratios of 1.15 (95% CI 1.06-1.23), 1.30 (95% CI 1.12-1.50) and 1.18 (95% CI 1.02-1.36) per interquartile range increase of 5.14 ppb in O3 for hospital admission, ICU admission and death, respectively. INTERPRETATION: Chronic exposure to air pollution may contribute to severe outcomes after SARS-CoV-2 infection, particularly exposure to O3.

A health political science for health promotion.

If health promotion as a field of change for human and ecological health is to maintain its urgency, it needs to continue building its policy credentials. This paper charts the development of policy as a concern for IUHE/IUHPE (International Union for Health Education/International Union for Health Promotion and Education) from the mid-1970s when 'health education policies' were prominent issues, to the launch of Healthy Public Policy (in the 1980s) and Health in All Policy (in the 2000s). We argue that solid conceptual and theoretical foundations exist to frame and develop the relevance and connectedness of health promotion more prominently. We start off with a brief introduction into (health) political science, and then illustrate the urgency of the argument with three case studies. The first takes a critical realist perspective on 'closing the gap' in Australian Indigenous populations. With recent evidence it demonstrates that the core of the policymaking process needs to re-align itself with an Indigenous narrative. The second case study reviews the politics of healthy urban planning and health equity in cities. Taking a critical theory institutionalist view, the case describes how the political and narrative parallels between urban theory and health equity have gone underexplored. With an explicit gaze to connect the two, the field could become a large and influential driver of enhanced health promotion and public health policy. The third case describes the languages, policy frames, and distinctions, in four urban/health paradigms. It shows that unconscious policy and practice bias exists in policy priorities and processes. We conclude with observations and recommendations on the role of health promotion as a conceptual realm and field of activity. We show that all health promoters should be aware of the political nature of their enterprise. Tools and analyses exist to help further action.

Factors influencing nurses' intention to care for patients with COVID-19: Focusing on positive psychological capital and nursing professionalism.

PURPOSE: It is necessary to identify factors that influence nurses' intention to care for coronavirus disease 2019 (COVID-19) patients to improve the quality of care during the pandemic. This study identifies factors that influence nurses' intention to care for COVID-19 patients, focusing on positive psychological capital and nursing professionalism. METHODS: This study adopted a descriptive correlational design. Data were collected between August 16 and August 30, 2020, through self-administered questionnaires from 148 bedside nurses caring for COVID-19 patients, from four hospitals designated for COVID-19 treatment. Modified versions of the Nursing Intention Questionnaire for SARS Patient Care, Psychological Capital Questionnaire, and Hall's Professional Inventory were used. The collected data were analyzed using stepwise multiple regression. RESULTS: In total, 165 questionnaires were distributed, and 148 questionnaires (89.7%) were included in the final analysis. Factors influencing nurses' intention to care were: age (30<: ß = .18, p = .026; ≥50: ß = .23, p = .005), department (ICU: ß = -.26, p = .001), sufficient clinical experience and skills to care for COVID-19 patients (sufficient: ß = .18, p = .019), and positive psychological capital (ß = .22, p = .044). The model's explanatory power (R2) was 48%. CONCLUSIONS: Strategies to increase nurses' positive psychological capital are necessary to improve nursing care quality by increasing intention to care when facing novel infectious diseases such as COVID-19. Furthermore, adequate education and training on managing novel infectious diseases should be implemented to provide nurses with relevant experience and skills regarding caring for patients infected with these diseases. Through various studies, strategies for improving nurses' positive psychological capital need to be suggested to improve the quality of care by increasing the nurses' intention to care during the emergence of a novel infectious disease, such as COVID-19. Additionally, adequate education and training on managing the novel infectious diseases, sufficient for the nurses to believe they have the experience and skills for caring for the infected patients, will be needed.

Computational Design of Potent D-Peptide Inhibitors of SARS-CoV-2.

Blocking the association between the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein receptor-binding domain (RBD) and the human angiotensin-converting enzyme 2 (ACE2) is an attractive therapeutic approach to prevent the virus from entering human cells. While antibodies and other modalities have been developed to this end, d-amino acid peptides offer unique advantages, including serum stability, low immunogenicity, and low cost of production. Here, we designed potent novel D-peptide inhibitors that mimic the ACE2 α1-binding helix by searching a mirror-image version of the PDB. The two best designs bound the RBD with affinities of 29 and 31 nM and blocked the infection of Vero cells by SARS-CoV-2 with IC50 values of 5.76 and 6.56 µM, respectively. Notably, both D-peptides neutralized with a similar potency the infection of two variants of concern: B.1.1.7 and B.1.351 in vitro. These potent D-peptide inhibitors are promising lead candidates for developing SARS-CoV-2 prophylactic or therapeutic treatments.

Nurses' Adaptations in Caring for COVID-19 Patients: A Grounded Theory Study.

As the novel coronavirus (COVID-19) pandemic continues, frontline nurses caring for COVID-19 patients are experiencing severe fatigue and mental stress. This study explored nurses' adaptation process in caring for COVID-19 patients and examined how nurses interact with the phenomenon using a grounded theory approach. The study aimed to develop a substantive theory and provide basic data with which to develop intervention programs that can support nurses caring for COVID-19 patients. Data were collected between 7 August and 31 October 2020, via face-to-face in-depth interviews with 23 nurses who had been caring for COVID-19 patients for six months or more at a nationally designated COVID-19 hospital. Sampling was started purposively and continued theoretically. Data analysis, performed using the method proposed by Strauss and Corbin, resulted in 13 main categories, the core one being "growing as a proficient nurse alongside comrades on the COVID-19 frontline". The study's results identify the nurses' adaptation process in caring for COVID-19 patients and their reactions to the circumstances around it. Ensuring that nurses can systematically cope with emerging infectious diseases requires regularly providing them with basic education on caring for patients with such diseases and strengthening professional education in order to develop nurses specializing in them. This study also recommends that a support system for work and childrearing be developed.

Safety and Effectiveness of an In-Hospital Screening Station for Coronavirus Disease 2019 in Response to the Massive Community Outbreak.

PURPOSE: To evaluate the results of operating an in-hospital coronavirus disease 2019 screening station on an outpatient basis and to identify the effectiveness and necessity of such a screening station. PATIENTS AND METHODS: This cross-sectional study included 1345 individuals who were tested for COVID-19 using real-time reverse transcription polymerase chain reaction (RT-PCR) at an in-hospital screening station on an outpatient basis. The subjects were healthcare workers (HCWs) with suspected COVID-19 symptoms or exposure to patients with confirmed COVID-19, caregivers at the hospital for complete enumeration, and patients who were scheduled to be admitted to a nonrestricted area in the hospital or to visit for outpatient treatment, but had suspected COVID-19 symptoms. The subjects were divided and compared as follows: HCW versus non-HCW groups and RT-PCR positive versus negative groups. RESULTS: A total of 140 had symptoms, 291 wanted to be tested, and 664 were asymptomatic but were screened. Seven subjects had positive results for COVID-19. Compared with the non-HCWs, the HCWs were younger and had a lower rate of underlying medical conditions. In addition, there were more women, individuals with exposure to confirmed cases, and individuals with symptoms or those who just wanted to be tested. The frequency of all symptoms was high among the HCWs. The results of the logistic regression analysis showed that the HCWs were significantly associated with the presence of symptoms, having an odds ratio of 23.317 (confidence interval, 15.142-35.907L; P < 0.001). The positive group had a high rate of exposure to patients with confirmed COVID-19 and had more subjects with symptoms or those who wanted to be tested. CONCLUSION: In-hospital screening stations are a relatively safe way to protect and support HCWs and to reduce and manage the spread of infection within the hospital effectively during an outbreak in the community.

A Review of "Optimal Fallow Period" Guidance Across Canadian Jurisdictions.

INTRODUCTION: Understanding how different countries have responded to mitigate the risk of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) transmission in dental offices is important. This article describes the different approaches taken towards optimal fallow periods in Canadian jurisdictions. METHODS: We searched publicly available information from dentist and dental hygiene regulator websites across the 10 provinces and 3 territories in Canada. We also searched for guidance documents on dental associations' websites or through personal communication with government officials. We extracted and tabulated information on fallow period recommendations or guidance, when available. RESULTS: Nine jurisdictions (6 provinces and all 3 territories) acknowledge or provide guidance on fallow periods following aerosol-generating procedures. Among those who have provided guidance regarding a fallow period, recommendations follow the Centers for Disease Control and Prevention guidance if the air changes per hour (ACH) in the dental operatory is known. CONCLUSION: The evidence for deciding on optimal fallow period is limited and still being explored, resulting in substantial variation across Canadian jurisdictions. A focus on developing scientific evidence relevant to dentistry and assimilating existing science is crucial to establishing consistency and uniformity in information to deliver safe oral health care services.

Engineering laminated paper for SARS-CoV-2 medical gowns.

The COVID-19 pandemic has highlighted the need for diversity in the market and alternative materials for personal protective equipment (PPE). Paper has high coatability for tunable barrier performance, and an agile production process, making it a potential substitute for polyolefin-derived PPE materials. Bleached and newsprint papers were laminated with polyethylene (PE) coatings of different thicknesses, and characterised for their potential use as medical gowns for healthcare workers and COVID-19 patients. Thicker PE lamination improved coating homogeneity and water vapour resistance. 49 GSM bleached paper with 16 GSM PE coating showed high tensile and seam strength, and low water vapour transmission rate (WVTR). Phi-X174 bacteriophage testing revealed that paper laminated with 15 GSM coating hinders virus penetration. This research demonstrates that PE laminated paper is a promising material for low cost viral protective gowns.